Oligonucleotide Synthesis: Methods and Applications (Methods in Molecular Biology)

e-Book & Magazine. October 11, 2010 by denisbul.


Oligonucleotide Synthesis: Methods and Applications (Methods in Molecular Biology)
Publisher: Humana Press | 2005 | ISBN: 1588292339 | Pages: 456 | PDF | Size: 11,6 MB

A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.

Download links:

Download File

Here you can download files online. Unlimited access to all sources file with one click direct download link!
Share

Comment